Coreldraw x5 slow3/21/2024 ![]() ![]() MO-based knock-down of TPC2 resulted in a dramatic attenuation of the Ca 2+ signals, whereas the introduction of TPCN2-MO and TPCN2 mRNA together partially rescued the Ca 2+ signaling signature. We used a transgenic line of zebrafish that expresses the bioluminescent Ca 2+ reporter, aequorin, specifically in skeletal muscle, in conjunction with morpholino (MO)-based and pharmacological inhibition of TPC2, in both intact embryos and isolated SMCs. Here, we show that the lysosomal two-pore channel 2 (TPC2) also plays a crucial role in generating, and perhaps triggering, these essential Ca 2+ signals, and thus contributes to the regulation of skeletal muscle myogenesis. ![]() We have recently characterized essential inositol 1,4,5-trisphosphate receptor (IP 3R) and ryanodine receptor (RyR)-mediated Ca 2+ signals generated during the differentiation of slow muscle cells (SMCs) in intact zebrafish embryos. ![]()
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